Protein_Domain
Strep-tag
Part:BBa_T2012:Design
Designed by: Reshma Shetty Group: iGEM07_Example (2009-08-11)
Strep-tag tail domain (WRHPQFGG)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This domain encodes the Strep-tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
Source
This is a synthetic sequence.
References
<biblio>
- Schmidt93 pmid=8433964
// this looks to be the original reference for the Strep-tag
- Schmidt pmid=8433964
- Voss pmid=9415448
- Skerra07 pmid=17571060
// includes brief overview of Strep-tag system
- Skerra00 pmid=11036648
// history of Strep-tag system
- Skerra99 pmid=10796988
- Skerra96 pmid=8636976
</biblio>