Protein_Domain
Strep-tag

Part:BBa_T2012

Designed by: Reshma Shetty   Group: iGEM07_Example   (2009-08-11)
Revision as of 19:47, 13 August 2009 by Rshetty (Talk | contribs)

Strep-tag tail domain (WRHPQFGG)

This is a Strep-tag tail domain designed to be at the C-terminus of a protein. Strep-tags are typically used for detection, purification and immobilization of proteins.

Strep-tag specifically binds to core streptavidin. As the Strep-tag binds reversibly to the same pocket of streptavidin where the natural ligand D-biotin is complexed, it can be used to purify corresponding fusion proteins on affinity columns with immobilized streptavidin.

Note that the Strep tag may only be used as a C-terminal tag. However, the Strep-tag II has been engineered for use as either an N- or C-terminal tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
//proteindomain/tail
Parameters
None