Protein_Domain
Strep-tag
Part:BBa_T2012
Designed by: Reshma Shetty Group: iGEM07_Example (2009-08-11)
Strep-tag tail domain (WRHPQFGG)
This is a Strep-tag tail domain designed to be at the C-terminus of a protein. Strep-tags are typically used for detection, purification and immobilization of proteins.
Strep-tag specifically binds to core streptavidin. As the Strep-tag binds reversibly to the same pocket of streptavidin where the natural ligand D-biotin is complexed, it can be used to purify corresponding fusion proteins on affinity columns with immobilized streptavidin.
Note that the Strep tag may only be used as a C-terminal tag. However, the Strep-tag II has been engineered for use as either an N- or C-terminal tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//proteindomain/affinity
//proteindomain/tail
//proteindomain/tail
None |