DNA

Part:BBa_K863201

Designed by: Julia Schirmacher   Group: iGEM12_Bielefeld-Germany   (2012-09-23)

3' UTR site of alcohol oxidase 1 gene (aox1)

3' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.

This part is a part of a shuttle vector (K863204) that could use for site directed recombination in the yeast P. pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 59


[edit]
Categories
//chassis/eukaryote
//chassis/eukaryote/pichia
//chassis/eukaryote/yeast
//direction/forward
//function/recombination
//plasmid/chromosomalintegration
//plasmid/component/other
Parameters
None