Coding
GFP_Freibu
Part:BBa_K863120
Designed by: Moritz Müller Group: iGEM12_Bielefeld-Germany (2012-09-20)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
GFP from Repressilator reporter in Standard 25 (Freiburg) See the untagged version for source information.
Usage and Biology
GFP (mut3b) [note that this part does not have a barcode]
Untagged version of gfp from Repressilator reporter. See the design page for more source information.
BioBrick has not yet been used. Built to be fused to binding domains for characterization.
Fluorescence wavelengths
Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -
- Excitation max - 501nm
- Emission max - 511nm
Latency
Cormack et al.Cormack report detectable fluorescence within 8 mins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 721 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 650
[edit]
Categories
Parameters
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
color | Green |
emission | 511 |
excitation | 501 |
lum | |
protein | GFPmut3b |
tag | None |