Reporter
CBDcex(J61
Part:BBa_K863104:Design
Designed by: Moritz Müller Group: iGEM12_Bielefeld-Germany (2012-09-21)
Cellulose binding Domain from C. Fimi with const. Promotor and GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
Illegal NgoMIV site found at 65
Illegal AgeI site found at 1136 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1047
Design Notes
This part is was made by a mixture of Freiburg-assembly of BBa_K863101 and BBa_K863121 and suffix insertion into BBa_J61101. Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter.
Source
The origin of the cellulose binding domain is a cloning vector of the fermantation group of Bielefeld University.