Composite
Part:BBa_K822004
Designed by: Jarle Pahr Group: iGEM12_NTNU_Trondheim (2012-09-25)
LacI generator (no promoter)
A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. For use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.
This part is a duplicate of BBa_K292006. The NTNU iGEM 2011 team found that the physical DNA available from the registry for that part did not match the registered sequence , and we have therefore remade it. Sequencing confirms that this part has the desired sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1168
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//function/regulation/transcriptional
direction | Forward |
function | Rep |
ligands | allolactose, IPTG |
protein | LacI |
swisspro | P03023 |
tag | LVA |