Part:BBa_K525998

Promoter T7 and RBS

T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from Escherichia coli but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, E. coli carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX).

Used to express e.g. BBa_K525123 - look there for typical induction profiles.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Improvement of K525998 by ZJU-CHINA 2017 Teams

The ZJU-CHINA 2017 Teams fulfilled the improvement of K525998 by inducing some mutation in the sequence. A series of promoters with different expression strength are constructed. The result can be seen by clicking the link below.

<a href=https://parts.igem.org/Part:BBa_K2207024>BBa_K2207024</a>

<a href=https://parts.igem.org/Part:BBa_K2207025>BBa_K2207025</a>

<a href=https://parts.igem.org/Part:BBa_K2207026>BBa_K2207026</a>

<a href=https://parts.igem.org/Part:BBa_K2207027>BBa_K2207027</a>

<a href=https://parts.igem.org/Part:BBa_K2207028>BBa_K2207028</a>

<a href=https://parts.igem.org/Part:BBa_K2207029>BBa_K2207029</a>

<a href=https://parts.igem.org/Part:BBa_K2207030>BBa_K2207030</a>

Contribution

Part name:BBa_K2382003
Group: iGEM17_CSMU_NCHU_Taiwan 2017
Author: TING-YU LIN
Summary: We tried to improve this promoter sequence by adding a Lac operator and making it RFC 10 compatible, since the Lac operator from pET-29a(+) has an illegal XbaI restriction enzyme site . The BBa_K525998 doesn't contain Lac operator sequence, and we managed to insert one in it so it would work better with the presence of LacI protein, making its regulation function better.
Documentation:

Functional Parameters: Austin_UTexas

BBa_K525998 parameters

Burden Imposed by this Part:

Burden Value: 0.2 ± 5.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.