Coding
CFP

Part:BBa_K411201:Design

Designed by: KO KUEI YUEH   Group: iGEM10_NYMU-Taipei   (2010-10-22)
Revision as of 15:41, 26 October 2010 by Blackrabbit (Talk | contribs)

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Cyan Fluorescent Protein (CFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Originated from A. victoria GFP. Has the same coding region as Part:BBa_E0021. We wanted a version of the ECFP that did not have an LVA tag.


Source

Two-part PCR of Part:BBa_E0022 to remove the LVA tag. Primers used:

  • The inner primers(our [http://2010.igem.org/Team:NYMU-Taipei/ "xFP LVA remover"]):
    • xFP_LVA_Remover_FP: 5' - gagctgtacaagaggccttaataatactagagccaggcatca - 3'
    • xFP_LVA_Remover_RP: 5' - tgatgcctggctctagtattattaaggcctcttgtacagctc - 3'
  • The outer primers (they include the biobrick prefix and suffix:
    • 5' - - 3'
    • 5' - - 3'


References