Difference between revisions of "Part:BBa K3892006"

Revision as of 08:18, 21 October 2021

EforRed

Red chromoprotein with strong RBS. This is iGEM21_Linkoping_Sweden's improvement of the BBa_K1073022. The BioBrick was improved by adding a stronger RBS and spacers (G-10 leader, BBa_K1758100 but the polyA sequence were removed and turned into BBa_K3892010) which increases the expression of the protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3892006 short</partinfo>
-Red chromoprotein with strong RBS. This is iGEM21_Linkoping_Sweden's improvement of the <partinfo>BBa_K1073022</partinfo>. The BioBrick was improved by adding a stronger RBS and spacers (G-10 leader, <partinfo>BBa_K1758100</partinfo> but the polyA sequence were removed and turned into <partinfo>BBa_K3892010</partinfo>) which increases the expression of the protein. This version of the protein has a glycine added after the start methionine to create a BamHI restriction enzyme site. This enables swapping of the RBS and promotor parts.
+Red chromoprotein with strong RBS. This is iGEM21_Linkoping_Sweden's improvement of the <partinfo>BBa_K1073022</partinfo>. The BioBrick was improved by adding a stronger RBS and spacers (G-10 leader, <partinfo>BBa_K1758100</partinfo> but the polyA sequence were removed and turned into <partinfo>BBa_K3892010</partinfo>) which increases the expression of the protein.
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