Composite

Part:BBa_K3447101

Designed by: Yu Ma, Yu He   Group: iGEM20_Jilin_China   (2020-10-21)
Revision as of 10:03, 27 October 2020 by Alswey (Talk | contribs)


light-on induced system

RelE is from Escherichia coli str. K-12, encoding RelE toxin.


Usage and Biology

In our project, we use this part to achieve the response of blue light and inhibit the growth of bacteria.

Arabinose induced RelE expression: BBa_K3447104

AHL induced RelE expression: BBa_K3447106

Design

Design Notes

We added some synonymous mutations to avoid part rules.



Source

We found this sequence data in GenBank.

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1882
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 587
    Illegal NgoMIV site found at 659
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 1259
    Illegal NgoMIV site found at 1552
    Illegal NgoMIV site found at 1646
    Illegal AgeI site found at 301
    Illegal AgeI site found at 1427
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1316
    Illegal BsaI.rc site found at 200


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Categories
Parameters
None