Composite

Part:BBa_K3447101

Designed by: Yu Ma, Yu He   Group: iGEM20_Jilin_China   (2020-10-21)


RelE, a kind of toxin protein

RelE is from Escherichia coli str. K-12, encoding RelE toxin.


Usage and Biology

In our project, we use this part to achieve the response of blue light and inhibit the growth of bacteria.

Arabinose induced RelE expression: BBa_K3447104

AHL induced RelE expression: BBa_K3447106

Design

Design Notes

We added some synonymous mutations to avoid part rules.



Source

We found this sequence data in GenBank.

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 467


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Parameters
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