RBS

Part:BBa_K3105111

Designed by: Katherin Vallejos Torres   Group: iGEM19_Uppsala_Universitet   (2019-08-26)


RBS with ribosome standby site

RBS (BBa_B0034) with an added ribosome standby site located upstream. The ribosome standby site was added with inverse PCR mutagenesis and showed increased expression of AsPink (BBa_K3105666) in the low copy number plasmid pSB3K3 compared to the unimproved RBS (BBa_B0034).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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