nMag - Light-activated protein
This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools. The negative Magnet (nMag) when irradiated by a blue light change through electrostatic interactions.
This part was engineered to be a building block of the standard BioBrick framework, and can be used with pMag (BBa_K2660009) to join different domains of a protein of interest when irradiated by blue light like N-cas9 (BBa_K2660006) and C-cas9 (BBa_K2660007) domains.
nMag- Light-activated protein
This part is inserted into plasmid, and the correct construction of this recombinant plasmid was confirmed by PCR identification and sequencing of the PCR products.