Coding
mCherry (L
Part:BBa_K2660003
Designed by: Nathan Vinicius Ribeiro Group: iGEM18_Unesp_Brazil (2018-10-08)
Revision as of 23:49, 12 October 2018 by Nathanribeiro (Talk | contribs)
mCherry codon optimized for Lactococcus lactis
mCherry protein reporter (BBa_J06504) codon-optimized for Lactococcus lactis. We weren't able to test this part in Lactococcus lactis but we tested in Bacillus subtilis and Escherichia coli and it works as well.
This part contains a RBS from Lactococcus lactis that is the target for the regulatory small RNA BBa_K2770006 (https://parts.igem.org/Part:BBa_K2270006).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/lactococcus
//collections/probiotics
//collections/probiotics/production
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/lactococcus
//collections/probiotics
//collections/probiotics/production
emission | 587 nm |
excitation | 610 nm |