Coding

Part:BBa_K2431002

Designed by: Asa M. Budnick   Group: iGEM17_NortheasternU-Boston   (2017-10-24)


BP100 AMP

This part codes for the BP100 Anti-Microbial Peptide. This AMP is known to disrupt the cell envelope of gram-negative bacteria. This part is designed to be used in a cell-free reaction containing T7 E. Coli cell extract.

Usage and Biology

Characterization


No Template Control: D & N

Above plates show the lack of bacterial killing due to PURExpress reactions without AMP template added. "N" denotes the normal reaction concentration and "D" represents a 1:4 dilution.


Part 11: BP100 (BBa_K2431002)

The above plate based bacterial killing assay shows the effective killing of BP100 produced via PURExpress. "L" and "S" represent promoters BBa_I712074 and BBa_I719005 respectively. "N" denotes the normal reaction concentration and "D" represents a 1:4 dilution. You can read more about experimental details on NortheasternU-Boston Experiments

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None