Coding

Part:BBa_K2431000

Designed by: Asa M. Budnick   Group: iGEM17_NortheasternU-Boston   (2017-10-24)


Microccocin C7 Anti-microbial Peptide Expression

This part contains the coding sequence for Micrococcin C7 an Antimicrobial peptide. The Part is flanked by 5'UTR and Terminator, allowing for easy amplification with overhanging primers containing a desired promoter sequence. Post amplification this part can be used directly in Cell Free Expression.

Usage and Biology

Characterization


No Template Control: D & N

Above plates show the lack of bacterial killing due to PURExpress reactions without AMP template added. "N" denotes the normal reaction concentration and "D" represents a 1:4 dilution.





Part 12: Micrococcin C7 (BBa_K2431000)

The above plate based bacterial killing assay shows the effective killing of Microccon C7 produced via PURExpress. "L" and "S" represent promoters BBa_I712074 and BBa_I719005 respectively. "N" denotes the normal reaction concentration and "D" represents a 1:4 dilution. You can read more about experimental details on NortheasternU-Boston Experiments


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None