RNA

Part:BBa_K1906001

Designed by: Wenbo Xu   Group: iGEM16_XJTLU-CHINA   (2016-07-31)
Revision as of 08:00, 20 October 2016 by Wenbo (Talk | contribs)


LtrB Group II intron Front Segment

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A Group II intron is a retrohoming element that can mediate the insertion of itself to a certain location of DNA. The Lactococcus lactis Ll.LtrB intron is a group II intron that found can target the ligated-exon sequence of the L. lactis ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.

<img src="XJTLU-intron-front.jpeg" /> Figure 1. The strucure of wildtype Ll.LtrB Group II intron. The intron contains a ORF that encodes a protein referred as LtrA, which is reseponsible for the mature and intergratation of intron RNA into DNA molecules.

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Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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