RNA

Part:BBa_K1906001

Designed by: Wenbo Xu   Group: iGEM16_XJTLU-CHINA   (2016-07-31)


LtrB Group II intron Front Segment

A Group II intron is a retro-homing element that can mediate the insertion of itself to a certain location of DNA. The Lactococcus lactis LtrB intron is a group II intron that can target the ligated-exon sequence of the L. lactis ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.

Figure 1. The structure of wild-type Ll.LtrB Group II intron. The intron contains an ORF that encodes a protein referred as LtrA, which is responsible for the mature and integration of intron RNA into DNA molecules.


This part is the first segment of non-transcriptional RNA, from the beginning of the RNA transcript to the start codon of LtrA.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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