Difference between revisions of "Part:BBa K157040"
 
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This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
 
This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
  
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It is derived from [[Part:BBa_J52017|BBa_J52017]].  There are a couple differences.  It lacks the 3' G in the BioBrick prefix (which should be present to comply with the BioBrick standard).  It is also missing an A in the BioBrick suffix between the SpeI site and NotI site.   
+
It is derived from [[Part:BBa_J52017|BBa_J52017]].  There are a couple differences.  It lacks the 3' G in the BioBrick prefix (which should be present to comply with the assembly standard 10).  It is also missing an A in the BioBrick suffix between the SpeI site and NotI site (which also should be present to comply with assembly standard 10).   
  
 
*'''[[User:Rshetty|RS]] 19:26, 23 November 2008 (UTC)''': This part has been split into a plasmid backbone and part and remapped to BBa_K157040-BBa_K157042.  BBa_K157040 is the plasmid backbone and BBa_K157042 is the part.  The physical DNA needs to be remapped to BBa_K157042.
 
*'''[[User:Rshetty|RS]] 19:26, 23 November 2008 (UTC)''': This part has been split into a plasmid backbone and part and remapped to BBa_K157040-BBa_K157042.  BBa_K157040 is the plasmid backbone and BBa_K157042 is the part.  The physical DNA needs to be remapped to BBa_K157042.

Latest revision as of 03:36, 9 December 2009

Eukaryotic terminator vector (very similar to pSB1AK8)

This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

It is derived from BBa_J52017. There are a couple differences. It lacks the 3' G in the BioBrick prefix (which should be present to comply with the assembly standard 10). It is also missing an A in the BioBrick suffix between the SpeI site and NotI site (which also should be present to comply with assembly standard 10).

  • RS 19:26, 23 November 2008 (UTC): This part has been split into a plasmid backbone and part and remapped to BBa_K157040-BBa_K157042. BBa_K157040 is the plasmid backbone and BBa_K157042 is the part. The physical DNA needs to be remapped to BBa_K157042.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 8
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444