Coding

Part:BBa_K1499400:Design

Designed by: Ian Hull   Group: iGEM14_StanfordBrownSpelman   (2014-10-09)
Revision as of 17:08, 24 October 2014 by Ianhull (Talk | contribs) (→‎Design Notes)

Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this gene was obtained from the Polistes dominula genome[1]. We codon-optimized it for expression in E. coli and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies.

Source

Polistes dominula genome.

References

Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB

Studies of amino acid composition of nest protein in other Polistes species: http://link.springer.com/article/10.1007/PL00001733 http://link.springer.com/article/10.1007/s101640070003 http://link.springer.com/article/10.1007/BF00997166 http://link.springer.com/article/10.1007/BF00982111