Composite

Part:BBa_K1499205:Design

Designed by: Raman Nelakanti   Group: iGEM14_StanfordBrownSpelman   (2014-10-09)
Revision as of 22:18, 2 November 2014 by Raman2004 (Talk | contribs) (→‎Design Notes)


MntH with amber stop codon with promoter for expression, no tRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 215
    Illegal NgoMIV site found at 1007
    Illegal NgoMIV site found at 1080
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The MntH was reisolated from Deinococcus radiodurans genomic DNA. Then, we used site-directed mutagenesis to introduce the stop codon in the gene.

Source

todo

References