Composite
Part:BBa_K1499205:Design
Designed by: Raman Nelakanti Group: iGEM14_StanfordBrownSpelman (2014-10-09)
MntH with amber stop codon with promoter for expression, no tRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 215
Illegal NgoMIV site found at 1007
Illegal NgoMIV site found at 1080 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The MntH was reisolated from Deinococcus radiodurans genomic DNA. Then, we used site-directed mutagenesis to introduce the stop codon in the gene. A TTG (leucine) codon was mutated to a TAG amber stop codon.
Source
Deinococcus radiodurans genomic DNA, then site-directed mutagenesis for a single base mutation.