Device

Part:BBa_K137132

Designed by: Josh Michener   Group: iGEM08_Caltech   (2008-10-23)

Lactose inducible GFP expression

A test construct to measure the response of the lactose-inducible promoter B4 with and without the expression of the mutant LacY permease. In the final system design, GFP will be replaced by the lysis cassette K137126 making K137131.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2132


[edit]
Categories
//function/regulation/transcriptional
Parameters
None