Generator

Part:BBa_K1319014

Designed by: Florian Gohr, Arne Zimmermann, Michael Osthege   Group: iGEM14_Aachen   (2014-10-05)
Revision as of 02:16, 18 October 2014 by Mosthege (Talk | contribs)

Constitutive expression of GFP-REACh2 fusion protein

This part expresses a E0040.K1319002 fusion protein (wtGFP-REACh1) behind a J23101 promoter. K1319002 is a dark quencher that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.

Usage and Biology

More information about the characterization of this part can be found on the part page of K1319002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


[edit]
Categories
Parameters
None