Coding

Part:BBa_K1152011

Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis   Group: iGEM13_Heidelberg   (2013-09-19)
Revision as of 23:20, 5 October 2013 by Herbstk (Talk | contribs)

Svp 4'-Phosphopanthetheinyl-transferase

EntD encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes It is the endogenous PPtases of Escherichia coli, located within the enterobactin pathway.

Figure 1: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 173
    Illegal NgoMIV site found at 413
    Illegal NgoMIV site found at 663
    Illegal AgeI site found at 573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 366


[edit]
Categories
Parameters
None