Part:BBa_K1152011

EntD 4'-Phosphopanthetheinyl-transferase

Figure 1: Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.

EntD (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (Figure 2). It is the endogenous PPtases of Escherichia coli, located within the enterobactin pathway.

Figure 2: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

Figure 3 shows the analytical restiction digest we performed for part validation:

Figure 3: Restriction digest with EcoRI, XbaI, SpeI, PstI.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]