Difference between revisions of "Part:BBa K1073024"

Revision as of 14:32, 30 September 2013

Constitutively expressed chromoprotein amilGFP

The device consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.

Usage and Biology

During cultivation, the chromoprotein amilGFP is expressed and stored intracellularly. The yellow color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter or selection marker to distinguish cells easily.

iGEM Team Braunschweig 2013:

Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP , eforRed (BBa_K1073022) and aeBlue (BBa_K1073020).

Right: Cell pellet of E. coli XL1 expressing amilGFP.

The yellow color of eforRed cannot only be seen by naked eye but it also fluoresce when excited. Thus it can be used to identify cells with fluorescent microscopy or FACS. For further information as well as absorption and emission spectra see experience page

iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed (BBa_K1073022) and amilGFP. Both chromoproteins fluoresce when excited by UV-light.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 16: / Line 16: @@
 '''Right:''' Cell pellet of ''E. coli XL1'' expressing amilGFP.
+The yellow color of eforRed cannot only be seen by naked eye but it also fluoresce when excited. Thus it can be used to identify cells with fluorescent microscopy or FACS. For further information as well as absorption and emission spectra see experience page
+[[File:Fluorescence.JPG |200px]]
+'''iGEM Team Braunschweig 2013:''' Supernatant after cell disruption containing chromoproteins of eforRed (<partinfo>BBa_K1073022</partinfo>) and amilGFP. Both chromoproteins  fluoresce when excited by UV-light.
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