Difference between revisions of "Part:BBa K1073024"

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<partinfo>BBa_K1073024 short</partinfo>
 
<partinfo>BBa_K1073024 short</partinfo>
  
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The device consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.  
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The device consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with a RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.  
  
  

Revision as of 11:44, 28 September 2013

Constitutively expressed chromoprotein amilGFP

The device consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.


Usage and Biology

During cultivation the chromoprotein amilGFP is expressed and stored intracellular. The yellow color of the protein can be seen by naked eye, thus you can use the chromoprotein as a reporter or selection marker and seperate cells easily.

Expressed chromoproteins.png AmilGFP pellet.png

iGEM Team Braunschweig 2013:

Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP , eforRed (BBa_K1073022) and aeBlue (BBa_K1073020).

Right: Cell pellet of E. coli XL1 expressing amilGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]