Part:BBa_J45004

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali

BBa_J45004 encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I derived from BSMT1 from Petunia x hybrida. BSMT1 catalyzes the conversion of benzoic acid to methyl benzoate and salicylic acid to methyl salicylate. Methyl benzoate has a floral smell and methyl salicylate has a wintergreen smell.

Usage and Biology

Substrate	pH optimum	Apparent Km for Substrate (μM)	kcat × 10-2 (s-1))	kcat/Km (mM-1⋅s-1))
SAMSA		1.93 ± 0.18	1.45 ± 0.024	7.65 ± 0.618
SAMBA		15.78 ± 1.34	0.75 ± 0.023	0.48 ± 0.047
Salicylic acid	7.0 to 8.0	51.55 ± 2.73	1.2 ± 0.16	0.23 ± 0.038
Benzoic acid	7.5	1273.2 ± 37	0.39 ± 0.04	0.003 ± 0.0003

<biblio>

1. Negre-2003 pmid=14630969

</biblio>

Contribution

Group: Valencia_UPV iGEM 2018 Authors: Adrián Requena Gutiérrez, Carolina Ropero

Summarize: we have created part BBa_K2656025. It is the BSMT1 coding sequence BBa_J45004 adapted into the Golden Gate assembly method. Thus, it is a basic part compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] grammar.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 663
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 204