Part:BBa_J428100
Interlab_NegC
Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.
Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.
It can be used as negative control for fluorescent measurements in general.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 71
Illegal NheI site found at 94 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Interlab 2022 Experiment 1 Devices
The following devices are used in the iGEM 2022 InterLaboratory Study for Experiment 1.
Note: that the well locations for samples of these devices referenced below are only for the iGEM 2022 Distribution.
| Device | Name | 2022 Distribution Location |
|---|---|---|
| BBa_J428100 | Negative Control | Kit Plate 1 Well 12M |
| BBa_I20270 | Positive Control | Kit Plate 1 Well 1A |
| BBa_J428112 | Test Device 1 (Green device) | Kit Plate 1 Well 14C |
| BBa_J428110 | Test Device 2 (Red mRFP1) | Kit Plate 1 Well 12O |
| BBa_J428111 | Test Device 3 (Red mCherry) | Kit Plate 1 Well 14A |
| BBa_J428101 | Test Device 4 (RiboJ ins. mCherry) | Kit Plate 1 Well 12I |
| BBa_J428108 | Test Device 5 (Dual constr. Blue and Red) | Kit Plate 1 Well 14E |
| BBa_J428106 | Test Device 6 (Dual constr. Green and Blue) | Kit Plate 1 Well 12G |
| None |