Part:BBa_J428100

Interlab_NegC

Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.

Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.

It can be used as negative control for fluorescent measurements in general.

Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.

Interlab 2022 Experiment 1 Device

• Negative Control for Experiment 1 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 1.

Interlab 2022 Experiment 2 Device

• Negative Control for Experiment 2 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 2.

Interlab 2022 Experiment 3 Device

• Negative Control for Experiment 3 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 3.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 71
  Illegal NheI site found at 94
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]