Composite

Part:BBa_K864102

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala_University   (2012-09-25)

B0034-SYFP2

This part is the Super Yellow Fluorescent Protein 2 (SYFP2) (BBa_K864100) with the strong BBa_B0034 RBS.

Usage and Biology

This part is usable as a reporter. The very strong fluorescence means that transcription can be detected at low levels. Colonies can clearly be seen under a UV table with most promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/chromoprotein/uppsala
Parameters
chassisCodon optimized for expression in E coli.
emission527 nm
excitation515 nm