RNA

Part:BBa_K2841514

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-13)


LacI Repressing gRNA

A gRNA which targets the LacIq promoter (BBa_K2841517) for the LacI protein (specifically designed for the Z1 cassette). When the gRNA is combined with a dCAS9 (BBa_K2841510) the LacI protein will be repressed, which means every Lac promoter will be induced. (Targets a LacIq promoter (BBa_K2841517) with the sequence: CGTTGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGA)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 102
    Illegal NgoMIV site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None