Device
dCAS9

Part:BBa_K2841510

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-13)


Streptococcus Pyogenes dCAS9

The dCAS9 protein used in the CRISPR repressor 2013 paper. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (BBa_K2841514) which directs it to a site of interest. The structure for the protein family has been solved.

dCAS9

The protein is shown in wheat colour BBa_K2841510. The RNA cis acting factor (for the dCAS9 protein to recognise the gRNA) is in grey BBa_K2841514. The gRNA's targeting region (what specifies the target sequence) is in green BBa_K2841514.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None