Part:BBa_J45500

Exponential phase wintergreen odor biosynthetic system

We assembled a complete, exponential phase dependent wintergreen odor biosynthetic system (BBa_J45500) by combining the salicylic acid (BBa_J45320) and exponential phase dependent wintergreen odor generators (BBa_J45181). BBa_J45500 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor. Wintergreen odor should only be produced in exponentially growing cells.

Usage and Biology

To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production. We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (BBa_J45320) based on the pchBA genes from Pseudomonas aeruginosa. The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Serino et al. previously demonstrated salicylate formation in E. coli using the pchBA genes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3762
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 389
  Illegal NgoMIV site found at 793
  Illegal NgoMIV site found at 918
  Illegal NgoMIV site found at 929
  Illegal NgoMIV site found at 1210
  Illegal NgoMIV site found at 1678
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3303