Coliroid
We have designed a bacterial system that is switched between different states by red light. The system consists of a synthetic sensor kinase that allows a lawn of bacteria to function as a biological film, such that the projection of a pattern of light on to the bacteria produces a high-definition (about 100 megapixels per square inch), two-dimensional chemical image.
Sample photos
Here are a selection of sample coliroids taken with the bacterial photography system.
- Macintosh HD-Users-nkuldell-Desktop-bacterialselfportrait.jpg
[http://openwetware.org/wiki/Jeff_Tabor Jeff Tabor] holding a coliroid.
Photo credit: Marsha Miller, University of Texas at Austin. Image courtesy of UT/UCSF. - UT HelloWorld.jpg
Hello World coliroid published in Levskaya et al., Nature, 2005.
- ColiroidEllington.png/200px-ColiroidEllington.png
This is a coliroid portait of Andy Ellington. You can compare it with the [http://www.icmb.utexas.edu/images/faculty/ellington.jpg real Andy]. Image courtesy of UT/UCSF.
- ColiroidFSM.jpg
This is a coliroid of the [http://venganza.org Flying Spaghetti Monster]. Image courtesy of UT/UCSF.
| http://openwetware.org/images/9/9a/Macintosh_HD-Users-nkuldell-Desktop-bacterialselfportrait.jpg | http://openwetware.org/images/8/83/UT_HelloWorld.jpg | http://openwetware.org/images/thumb/d/d1/ColiroidEllington.png/200px-ColiroidEllington.png | http://openwetware.org/images/6/68/ColiroidFSM.jpg |
| [http://openwetware.org/wiki/Jeff_Tabor Jeff Tabor] holding a coliroid. Photo credit: Marsha Miller, University of Texas at Austin. Image courtesy of UT/UCSF. |
Hello World coliroid published in Levskaya et al., Nature, 2005. | This is a coliroid portait of Andy Ellington. You can compare it with the [http://www.icmb.utexas.edu/images/faculty/ellington.jpg real Andy]. Image courtesy of UT/UCSF. | This is a coliroid of the [http://venganza.org Flying Spaghetti Monster]. Image courtesy of UT/UCSF. |
Device implementation
This system consists of two devices.
- A light sensor which takes red light as an input and produces PoPS as an output.
- A color generator which takes a PoPS signal as input and generate color as an output.
Diagram courtesy of [http://openwetware.org/wiki/Drew_Endy Drew Endy].
Parts implementation
This diagram shows the list of parts that make up each of the two devices.
Diagram courtesy of [http://openwetware.org/wiki/Drew_Endy Drew Endy].
For details on system implementation, see the reference at the bottom of this page.
| Name | Type | Description | Length | Status |
|---|---|---|---|---|
| BBa_B0034 | RBS | RBS (Elowitz 1999) -- defines RBS efficiency | 12 | In stock |
| BBa_E0033 | Reporter | LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega) | 353 | In stock |
| BBa_I15008 | Coding | heme oxygenase (ho1) from Synechocystis | 751 | In stock |
| BBa_I15009 | Coding | phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis | 775 | In stock |
| BBa_I15010 | Coding | cph8 (Cph1/EnvZ fusion) | 2263 | In stock |
| BBa_K098010 | Generator | HO-pcyA, low expression | 1531 | It's complicated |
| BBa_K098011 | Generator | E. coli ompR, expressed at low level | 917 | It's complicated |
| BBa_K1479004 | RBS | Just for upload test | 431 | Not in stock |
| BBa_K1587008 | Device | Red light biosensor | 2288 | It's complicated |
| BBa_K1660004 | Composite | PcyA+Ho1 | 1585 | It's complicated |
| BBa_K1660005 | Reporter | RFP controlled by the PompR promoter | 978 | In stock |
| BBa_K1660006 | Composite | J23100-B0034-cph8 | 2299 | Not in stock |
| BBa_K1660007 | Composite | PompR-6N-gp35-Terminator | 1773 | It's complicated |
| BBa_K2656011 | RBS | Medium Ribosome Binding Site B0034 | 22 | It's complicated |
| BBa_K338029 | Composite | +OmpR, +(CinR-HSL) Double Promoter | 347 | It's complicated |
| BBa_K592000 | Coding | cph8 (Cph1/EnvZ fusion) | 2238 | In stock |
| BBa_M30109 | Composite | Light responsive system, dual regulation | 4333 | It's complicated |
| BBa_M45047 | Signalling | Estrogen Activated GFP | 1035 | Not in stock |
| BBa_R0082 | Regulatory | Promoter (OmpR, positive) | 108 | In stock |
| System design and implementation by [http://openwetware.org/wiki/User:Levskaya Anselm Levskaya], Aaron Chevalier, [http://openwetware.org/wiki/Jeff_Tabor Jeff Tabor], [http://openwetware.org/wiki/User:LLavery Laura Lavery], Matthew Levy, Eric Davidson, Alexander Scouras, [http://ellingtonlab.org/ Andy Ellington], Ed Marcotte, and [http://www.voigtlab.ucsf.edu/ Chris Voigt]. |
References
<biblio>
- Levskaya pmid=16306980
</biblio>
- [http://openwetware.org/wiki/BE.109:Systems_engineering MIT Biological Engineering laboratory module] on bacterial photography developed by [http://openwetware.org/wiki/Natalie_Kuldell Natalie Kuldell]
- [http://openwetware.org/wiki/LightCannon Instructions] for how to build a "light cannon" for use in bacterial photography.