User contributions
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- 07:32, 21 October 2019 (diff | hist) . . (+76) . . Part:BBa K2985004 (current)
- 07:30, 21 October 2019 (diff | hist) . . (+256) . . Part:BBa K2985004
- 07:24, 21 October 2019 (diff | hist) . . (+6) . . Part:BBa K2985160 (current)
- 07:24, 21 October 2019 (diff | hist) . . (+6) . . Part:BBa K2985002 (current)
- 07:20, 21 October 2019 (diff | hist) . . (+174) . . Part:BBa K2985002
- 07:16, 21 October 2019 (diff | hist) . . (+4) . . Part:BBa K2985002
- 07:15, 21 October 2019 (diff | hist) . . (+304) . . Part:BBa K2985002
- 07:11, 21 October 2019 (diff | hist) . . (+174) . . Part:BBa K2985160
- 07:06, 21 October 2019 (diff | hist) . . (+382) . . Part:BBa K2985160
- 03:44, 20 October 2019 (diff | hist) . . (-2) . . Part:BBa K2985002
- 03:43, 20 October 2019 (diff | hist) . . (+2,603) . . Part:BBa K2985002
- 03:36, 20 October 2019 (diff | hist) . . (+97) . . N File:Jiangnan-China yerP-figure 4.png (Fig. 4 the morphology of Bacillus subtilis after transformation at higher surfactin concentration) (current)
- 03:36, 20 October 2019 (diff | hist) . . (+88) . . N File:Jiangnan-China yerP-figure 3.png (Figure 3 Morphology of wild-type Bacillus subtilis 168 at higher surfactin concentration) (current)
- 03:36, 20 October 2019 (diff | hist) . . (+4) . . N File:Jiangnan-China yerP-figure 5.jpeg (HPLC) (current)
- 03:35, 20 October 2019 (diff | hist) . . (+97) . . N File:Jiangnan-China yerP-figure 1.jpeg (Figure 1: the red gene is the yerP gene, which we inserted. Its location is between yerO and yerQ) (current)
- 03:35, 20 October 2019 (diff | hist) . . (+138) . . N File:Jiangnan-China yerP-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 3198 bp, which is consistent with the target length.) (current)
- 03:31, 20 October 2019 (diff | hist) . . (0) . . File:Jiangnan-China ycxA-figure 1.jpeg (JNUsdq uploaded a new version of File:Jiangnan-China ycxA-figure 1.jpeg) (current)
- 03:30, 20 October 2019 (diff | hist) . . (+7) . . Part:BBa K2985004
- 03:27, 20 October 2019 (diff | hist) . . (+3,433) . . Part:BBa K2985004
- 03:20, 20 October 2019 (diff | hist) . . (+88) . . N File:Jiangnan-China ycxA-figure 7.jpeg (after transformation, the colony is relatively large and the hemolysis circle is larger.) (current)
- 03:20, 20 October 2019 (diff | hist) . . (+62) . . N File:Jiangnan-China ycxA-figure 4.jpeg (co-culture of wild type bacillus subtilis and Escherichia coli) (current)
- 03:19, 20 October 2019 (diff | hist) . . (+67) . . N File:Jiangnan-China ycxA-figure 6.jpeg (relatively small colonies producing surfactin before transformation) (current)
- 03:19, 20 October 2019 (diff | hist) . . (+73) . . N File:Jiangnan-China ycxA-figure 5.jpeg (co-culture of Bacillus subtilis and Escherichia coli after transformation) (current)
- 03:18, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China ycxA-figure 3.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1227bp, consistent with the target length.) (current)
- 03:18, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China ycxA-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1104bp, consistent with the target length.) (current)
- 03:16, 20 October 2019 (diff | hist) . . (+155) . . N File:Jiangnan-China ycxA-figure 1.jpeg (Figure 1: upstream homologous arm (742bp) + SPC resistance gene (1104bp) + P43 promoter (678 / 56bp) + ycxA (1227bp) + downstream homologous arm（646bp）)
- 03:05, 20 October 2019 (diff | hist) . . (-10) . . Part:BBa K322921:Experience (current)
- 03:04, 20 October 2019 (diff | hist) . . (-5) . . Part:BBa K322921:Experience
- 03:04, 20 October 2019 (diff | hist) . . (+3) . . Part:BBa K322921:Experience
- 03:01, 20 October 2019 (diff | hist) . . (+46) . . Part:BBa K322921:Experience
- 02:59, 20 October 2019 (diff | hist) . . (+5) . . N File:Jiangnan-China sacB-figure 7.jpeg (table) (current)
- 02:53, 20 October 2019 (diff | hist) . . (+20) . . Part:BBa K143013
- 02:51, 20 October 2019 (diff | hist) . . (-97) . . Part:BBa K322921:Experience
- 02:50, 20 October 2019 (diff | hist) . . (+17) . . Part:BBa K322921:Experience
- 02:48, 20 October 2019 (diff | hist) . . (+3) . . Part:BBa K322921:Experience
- 02:46, 20 October 2019 (diff | hist) . . (+3,555) . . Part:BBa K322921:Experience
- 02:39, 20 October 2019 (diff | hist) . . (+37) . . N File:Jiangnan-China sacB-figure 6.png (Enzyme activity-sucrose concentration) (current)
- 02:37, 20 October 2019 (diff | hist) . . (+41) . . N File:Jiangnan-China sacB-figure 4.jpeg (medium without expression of pullulanase) (current)
- 02:37, 20 October 2019 (diff | hist) . . (+42) . . N File:Jiangnan-China sacB-figure 5.jpeg (culture medium with pullulanase expression) (current)
- 02:36, 20 October 2019 (diff | hist) . . (+116) . . N File:Jiangnan-China sacB-figure 3.jpeg (An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase.) (current)
- 02:36, 20 October 2019 (diff | hist) . . (+173) . . N File:Jiangnan-China sacB-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation of positive cloned DNA. The product has a signal band at 534bp respectively, which is consistent with the target length.) (current)
- 02:35, 20 October 2019 (diff | hist) . . (+80) . . N File:Jiangnan-China sacB-figure1.jpeg (sacB promoter (534bp) + RBS (BBa_K2985008) + pul gene (BBa_K2985009) + screening) (current)
- 02:26, 20 October 2019 (diff | hist) . . (+1,145) . . Part:BBa K322921:Experience
- 02:21, 20 October 2019 (diff | hist) . . (-1) . . Part:BBa K143013
- 02:17, 20 October 2019 (diff | hist) . . (+1) . . Part:BBa K143013
- 02:17, 20 October 2019 (diff | hist) . . (+62) . . N File:Jiangnan-China P43-figure10.jpeg (P43 promoter (56bp) + pullulanase gene (bba_k2985009) + screen) (current)
- 02:16, 20 October 2019 (diff | hist) . . (-12) . . Part:BBa K143013
- 02:14, 20 October 2019 (diff | hist) . . (+2,912) . . Part:BBa K143013
- 02:01, 20 October 2019 (diff | hist) . . (+20) . . N File:Jiangnan-China P43-figure 7.png (Enzyme activity-Time) (current)
- 02:01, 20 October 2019 (diff | hist) . . (+7) . . N File:Jiangnan-China P43-figure 6.png (OD-Time) (current)
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