Generator

Part:BBa_K395600:Design

Designed by: Toshitaka Matsubara   Group: iGEM10_Tokyo_Tech   (2010-10-04)
Revision as of 19:50, 27 October 2010 by Matsubara (Talk | contribs) (→‎Source)

Apple fragrance enzyme (MpAAT1) generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
    Illegal BamHI site found at 1635
    Illegal XhoI site found at 1675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 205
    Illegal AgeI site found at 1419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag


Source

The gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala).

References