Generator

Part:BBa_K395600:Design

Designed by: Toshitaka Matsubara   Group: iGEM10_Tokyo_Tech   (2010-10-04)

Apple fragrance enzyme (MpAAT1) generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
    Illegal BamHI site found at 1635
    Illegal XhoI site found at 1675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 205
    Illegal AgeI site found at 1419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag


Source

The gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala).

References

Edwige J. F. Souleyre et al. An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor, FEBS Journal, 272, 3132–3144 (2005)