Part:BBa_K300005:Experience

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Applications of BBa_K300005

It was used do design the following BioBrick measurement systems:

• BBa_K300086
• BBa_K300088
• BBa_K300090
• BBa_K300091
• BBa_K300092
• BBa_K300099

to test these parts:

• BBa_K300002
• BBa_K300093
• BBa_K300094
• BBa_K300095
• BBa_K300084
• BBa_K300097

respectively. All of the tested parts are synthetic fusion tags whose activity could be measured by assembling a promoter with RBS upstream and a tail domain with terminator downstream, thus yielding the measurement systems. BBa_K300005 was used as a tail domain with terminator downstream. It has been assembled to test the correct folding of the resulting fusion protein by measuring the GFP and to test the affinity tag performance with a proof of concept protein.

In all of the measurement parts, GFP could be successfully detected in bacteria harbouring the measurement parts in high copy plasmids. In this condition, GFP was detected by using an excitation filter at 485nm and an emission filter at 540nm in a Infinite F200 microplate reader (Tecan).

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