![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Pveg Xyle
Part:BBa_K316005
Designed by: IC 2010 Team Group: iGEM10_Imperial_College_London (2010-10-22)
Functional XylE under Pveg promoter, with double terminator
Enables characterization and comparison with alternative promoters e.g. BBa_J23101 Designed for characterisation of XylE in Bacillus subtilis where Pveg is a useful constitutive promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 442
Illegal NgoMIV site found at 614
Illegal AgeI site found at 965 - 1000COMPATIBLE WITH RFC[1000]
Part Characterisation
Characterisation data was obtained using GFP-XylE constructs BBa_K316008 and XylE under two different promoters: B. subtilis derived Pveg BBa_K316005 and J23101 BBa_K316004 from E. coli. These are described in our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the relevant parts pages.
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Categories
Parameters
n/a | Functional XylE under Pveg promoter, with double terminator |