Reporter

Part:BBa_K316004

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-20)

Functional XylE under J23101 promoter, with double terminator

Standard constitutive E.coli promoter BBa_J23101 combined with BBa_K316003 for comparative characterisation of gene activity (catechol breakdown into 2,4 hydromuconic semialdehyde yellow product).

For more information about XylE, it's substrate and spectrophotometric assays, please see BBa_K316003 or our [http://2010.igem.org/Team:Imperial_College_London/Results wiki results section]


Safety

The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the [http://www.sciencelab.com/msds.php?msdsId=9927131 Material Safety Data Sheet].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal NgoMIV site found at 552
    Illegal AgeI site found at 903
  • 1000
    COMPATIBLE WITH RFC[1000]


Part Characterisation

Characterisation data was obtained for XylE BBa_K316003. In addition constructs under two different promoters: J23101-XylE BBa_K316004 from E. coli was used to categorise B. subtilis derived Pveg-XylE BBa_K316005. Also GFP-XylE constructs BBa_K316007 were tested to determine the effectiveness of inhibition of XylE activity by attachment of GFP. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.

[edit]
Categories
Parameters
n/aFunctional XylE under J23101 promoter, with double terminator