Regulatory
AraC
Part:BBa_R0080
Designed by: Sara Neves (Fighting Darwins) Group: Antiquity (2004-01-27)
Promoter (AraC regulated)
AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
Usage and Biology
araC binding region from E.coli
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 103
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
biology | |
control | araC |
direction | Forward |
negative_regulators | |
o_h | |
o_l | |
positive_regulators | 1 |