![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K342002:Design
Designed by: Anne Chevrel Group: iGEM10_INSA-Lyon (2010-10-24)
Phasin-Phasin Intein (for silver standard fusion)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1693
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1279
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a self cleavable fusion protein. It has been designed thanks to the results of the publication Banki et al. The second phasin increases the rate of purified protein during the step of granules extraction.The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence
The phasin sequence is the same as the biobrick BBa_K208001 from the Utah 2009 team.
The sequencing of this part is difficult because of the repetition of phasin's sequence.
Source
Ligation of two synthetized parts : Phasin and Phasin-Intein