Part:BBa_K208001
Phasin (PhaP)
This is a Silver-fusion compatible BioBrick part that codes for phasin. The Silver-fusion compatibility standard was used so that this part could be fused with small signal peptides, which target proteins for export out of the cytoplasm. This part is functional both in fusion and non-fusion applications.
Usage and Biology
Phasin (PhaP) is a low-molecular weight protein that plays a role in PHA granule formation by physically binding to the PHA granule surface (York, 2001). The specific purpose of phasin production is not completely understood (York 2002), although some of the affects of the phasin/PHA interaction have been studied. York et al (2001) determined that the production of phasin is dependent on PHA accumulation. Specifically, it is suggested that phasin expression requires the presence of PHA synthase (York, 2001). Maehara et al (1999) observed that the level of PHA accumulation substantially decreases and the size of PHA granules increases when phasin is either absent or regulated by a repressor, PhaR. Therefore, PHA production levels are enhanced in the presence of phasin due to an increased granule surface-to-volume ratio (York 2001; Maehara 1999).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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