Coding

Part:BBa_K342002:Design

Designed by: Anne Chevrel   Group: iGEM10_INSA-Lyon   (2010-10-24)
Revision as of 14:41, 25 October 2010 by Achevrel (Talk | contribs) (→‎Design Notes)

Phasin-Phasin Intein (for silver standard fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1693
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1279
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been designed thanks to the results of the publication Banki et al. The two phasins

The phasin sequence is the same as the biobrick BBa_K208001 from the Utah 2009 team.

The sequencing of this part is difficult because of the repetition of phasin's sequence.

Source

Ligation of two synthetized parts : Phasin and Phasin-Intein

References