Coding

Part:BBa_K305002:Design

Designed by: Dennis Claessen, Maarten van den Nieuwenhof, Ramon Sieber, Arend Jan Suk   Group: iGEM10_Groningen   (2010-09-27)
Revision as of 08:24, 25 October 2010 by Jorrithillebrand (Talk | contribs) (→‎References)

Chaplin C (chpDC) with modified signal sequence and sortase recognition motive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536


Design Notes

Ordered as construct from Mr. Gene after genomic sequence had been identified and signal sequence + sortase motive had been modified.


Source

codon optimized for B. subtilis

References

Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726