Coding

Part:BBa_K305002:Design

Designed by: Dennis Claessen, Maarten van den Nieuwenhof, Ramon Sieber, Arend Jan Suk   Group: iGEM10_Groningen   (2010-09-27)

Chaplin C (chpDC) with modified signal sequence and sortase recognition motive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536


Design Notes

Ordered as construct from Mr. Gene after genomic sequence had been identified and signal sequence + sortase motive had been modified.


Design Notes

The RBS sequence used in this part can be found in several B. subtilis genes and is known to efficiently initiate translation. The spacer sequence between the RBS and start codon originates form the B. subtilis wapA gene. The CDS was optimized for B. subtilis by the GenScript OptimumGeneTM software (see images).

Frequency of optimal codons before optimization. The x-axis 100 value is set for the codon with the highest usage frequency for a given amino acid in B. subtilis.
Frequency of optimal codons after optimization. The x-axis 100 value is set for the codon with the highest usage frequency for a given amino acid in B. subtilis.


References

Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726