Project

Part:BBa_K404117

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-10-11)
Revision as of 03:26, 25 October 2010 by Bea (Talk | contribs)

[AAV2]-left-ITR_pCMV_betaglobin

[AAV2-left-ITR_pCMV_betaglobin]
Freiburg10 Vectorplasmid precursors 4.png
BioBrick Nr. BBa_K404117
RFC standard RFC 10
Requirement pSB1C3
Source synthetic
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]


Constructing viral particles encapsidating the transgene expression cassette flanked by the inverted terminal repeats (ITRs, BBa_K404100 and BBa_K404101) can be used in several applications, such as efficient tumor killing or quantification assays.
The plasmid containing the desired gene of interest is cotransfected with the RepCap plasmid (BBa_K404001, BBa_K404002 or BBa_K404003) and the pHelper plasmid. Several assembly steps have to be performed in order to obtain the fully assembled vectorplasmid.
Facilitating the cloning steps the iGEM team Freiburg_Bioware 2010 provide the 3' nucleotide components in respect to the desired gene of interest which is the left inverted terminal repeat (BBa_K404100) followed by the CMV promoter (BBa_K404102) and a putative enhancer-element (BBa_K404107).










Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//function/regulation/transcriptional
//viral_vectors/aav
//viral_vectors/aav/vector_plasmid
Parameters
None