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Part:BBa_K329017

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-24)
Revision as of 16:16, 24 October 2010 by Registry (Talk | contribs)

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Population counter Tetracycline resistant - PmS-MTet-LuxI

This part is one of our population counters. When IntI1 (BBa_K329014) is expressed, it excises the double terminator flanked by attC sites (BBa_J99001) and triggers the expression of TetA(C) (with a medium RBS, BBa_K329000) because of the constitutive promoter upstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
    Illegal NheI site found at 29
    Illegal NheI site found at 1293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3004
    Illegal BamHI site found at 1439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1465
    Illegal NgoMIV site found at 1833
    Illegal NgoMIV site found at 1993
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None